b. The loxP-PGK-gb2-neo-loxP template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of … English. 2) View the complete definition of any word or phrase using the toolbar dictionary. adj., adj re´gional. loxP flanked, Pro and Euk. ‘Seated in the dock, flanked by prison officers, he adopted his customary stance - shoulders slouched forward, head bowed.’ ‘Two black leather sofas flanked a low table in front of the receptionist, and Wendy chose one.’ ‘Every computer desk I've had until recently was flanked … It occurs commonly among lower plants and invertebrate animals (particularly rotifers, aphids, ants, wasps, and bees) and rarely among higher vertebrates. Spliceosome-associated proteins and splicing mediators are colored blue and green, respectively. WikiMatrix. WikiMatrix. A lateral part or side: the flank of a mountain. To brush one's hair, usually their fringe, backwards. It mediates higher transcription efficiency than the generally u,biological,biology supply,biology supplies,biology product RNAPII and chromatin components are colored purple in (A) and (B), respectively. The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in various bacteria and fungi. Thus the natural DNA-repair mechanisms of the cell can be used to insert the desired genetic material, editing the genome of a target cell with high-precision. 4. a. 3) Store a history of words you've looked up, so you can practice them later. These shields are flanked by two male figures who function as bearers of a coat of arms. The answer should be written using the space allocated below each question. region [re´jun] a plane with more or less definite boundaries; called also regio. 3. Rep and Cap are translated to produce multiple distinct proteins (Rep78, Rep68, Rep52, Rep40 - required for the AAV life cycle; VP1, VP2, VP3 - capsid proteins). Although recent years have seen numerous advances towards this goal, Synthetic Biology is still mostly a microbial-centric discipline, and high performance genetic circuits are currently lacking in mammalian cells. n. 1. At the end of the process the integrated provirus is flanked by a direct repeat of cell sequences, similar to the repeats that flank DNA transposons. Usually, people would prefer not to be seen whilst their fringe is flanked. 2. What does it mean when DNA is 'flanking'? Site-specific DNA … Thanks! How to use flank in a sentence. Flank definition: An animal's flank is its side, between the ribs and the hip. See more. 2. Find more ways to say flanked, along with related words, antonyms and example phrases at Thesaurus.com, the world's most trusted free thesaurus. “DNA cloning is a molecular biology technique which is used for the creation of exact copies or clones of a particular gene or DNA.” DNA cloning is the process of making multiple copies of a particular segment of DNA. The right or left side of a military formation: an attack on both flanks. Gene cassettes are segments of DNA with one or two open reading frames (ORFs) that lack promoters and are flanked by 59-base elements (59-be, also known as attC sites). flank. Task set B contains 2 essay questions (4 in the final exam). The east face of the bath had an ornate entrance in its center, flanked by excedrae. This result indicated that we had succeeded in tracing the descendent cells of vasa -expressing early-stage germ cells with GFP via a Cre/ loxP -mediated reporter system. These ITRs base pair to allow for synthesis of the complementary DNA strand. This is the meaning of flox: flox (English)Origin & history I Abbreviation of "flanked by loxP" Verb flox (third-person singular simple present floxes, present participle floxing, simple past and past participle floxed) . The 5′ flanking region contains the promoter, and may contain enhancers or other protein binding sites. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment that was obtained with a 1- or 3-step PCR protocol and that carries an antibiotic cassette flanked by a region homologous to the target locus into a strain that … A cut of meat from the flank of an animal. flank synonyms, flank pronunciation, flank translation, English dictionary definition of flank. Or phrase using the toolbar dictionary colored blue and green, respectively on both flanks their... Been used to inactivate chromosomal genes in various bacteria and fungi Double-click word. 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